A SIMPLE AND EFFICIENT VITRIFICATION METHOD FOR IN-STRAW DILUTION AND DIRECT TRANSFER OF BOVINE EMBRYOS

作者：Zhang Youwen; Fu Xiangwei; Chen Long; Feng Chuntao; Bi Jianghua; Mo Xianhong; Cheng Keren; Zhang Rina; Li Shujing; Zhu Shien^*

来源：Cryo-Letters, 2015, 36(6): 392-398.

• Bovine
• IVF
• blastocyst
• vitrification
• in-straw dilution
• embryo transfer

BACKGROUND: An easy and user friendly protocol that produces consistent results will facilitate the commercial application of embryo vitrification technology in the field. OBJECTIVE: This study was designed to develop a simple and efficient vitrification, in-straw dilution and direct transfer method for bovine embryos. METHODS: After being vitrified and in-straw thawed, in vivo-derived and in vitro-produced bovine embryos were subjected to in vitro culture or embryo transplantation. RESULTS: There were no significant differences (P>0.05) in survival rates (100.0% vs. 93.9%) and expansion rates (93.8% vs. 87.5%) between in vivo-derived and in vitro-produced blastocysts after vitrification and in-straw dilution. And there was also no significant difference (P>0.05) in conception rates (56.5% vs. 58.8%) after ET between cryopreserved and fresh in vivo-derived blastocysts. CONCLUSION: Vitrification using EG-based vitrification solution and in-straw dilution with PBS-based diluent is a simple and efficient method for cryopreservation and direct transfer of bovine embryos.

• 出版日期2015-12
• 单位中国农业大学