A Conductometric Indium Oxide Semiconducting Nanoparticle Enzymatic Biosensor Array

作者：Lee Dongjin; Ondrake Janet; Cui Tianhong^*

来源：Sensors, 2011, 11(10): 9300-9312.

DOI：10.3390/s111009300

• biosensor array
• nanoparticle
• conductometric sensor
• microsensor array
• glucose sensor

We report a conductometric nanoparticle biosensor array to address the significant variation of electrical property in nanomaterial biosensors due to the random network nature of nanoparticle thin-film. Indium oxide and silica nanoparticles (SNP) are assembled selectively on the multi-site channel area of the resistors using layer-by-layer self-assembly. To demonstrate enzymatic biosensing capability, glucose oxidase is immobilized on the SNP layer for glucose detection. The packaged sensor chip onto a ceramic pin grid array is tested using syringe pump driven feed and multi-channel I-V measurement system. It is successfully demonstrated that glucose is detected in many different sensing sites within a chip, leading to concentration dependent currents. The sensitivity has been found to be dependent on the channel length of the resistor, 4-12 nA/mM for channel lengths of 5-20 mu m, while the apparent Michaelis-Menten constant is 20 mM. By using sensor array, analytical data could be obtained with a single step of sample solution feeding. This work sheds light on the applicability of the developed nanoparticle microsensor array to multi-analyte sensors, novel bioassay platforms, and sensing components in a lab-on-a-chip.

• 出版日期2011-10