Analysis-Preserving Video Microscopy Compression via Correlation and Mathematical Morphology

作者：Shao Chong^*; Zhong Alfred; Cribb Jeremy; O**orne Lukas D; O'Brien E Timothy III; Superfine Richard; Mayer Patel Ketan; Taylor Russell M II

来源：Microscopy Research and Technique, 2015, 78(12): 1055-1061.

DOI：10.1002/jemt.22584

• biomedical image processing
• data compression
• image analysis

The large amount video data produced by multi-channel, high-resolution microscopy system drives the need for a new high-performance domain-specific video compression technique. We describe a novel compression method for video microscopy data. The method is based on Pearson's correlation and mathematical morphology. The method makes use of the point-spread PSF) in the microscopy video acquisition phase. We compare our method to other lossless compression methods and to lossy JPEG, JPEG2000, and H.264 compression for various kinds of video microscopy data including fluorescence video and brightfield video. We find that for certain data sets, the new method compresses much better than lossless compression with no impact on analysis results. It achieved a best compressed size of 0.77% of the original size, 253 smaller than the best lossless technique (which yields 20% for the same video). The compressed size scales with the video's scientific data content. Further testing showed that existing lossy algorithms greatly impacted data analysis at similar compression sizes.

• 出版日期2015-12