Molecular cloning of cyclin B transcript with an unusually long 3' untranslation region and its expression analysis during oogenesis in the Chinese mitten crab, Eriocheir sinensis

作者：Fang Jun Jiang; Qiu Gao Feng^*

来源：Molecular Biology Reports, 2009, 36(6): 1521-1529.

DOI：10.1007/s11033-008-9346-9

• Cyclin B
• cDNA cloning
• Differential expression
• Oogenesis
• 3 ' untranslated regions

The meiotic maturation of oocyte in animals is regulated by maturation promotion factor (MPF), a complex of Cdc2 and cyclin B. Although the role of MPF during oocyte maturation has been well studied in a wide variety of eukaryotic organisms, little is known for crustacean species. In this study, a full-length cDNA of cyclin B was cloned from the Chinese mitten crab using degenerate RT-PCR and RACE methods. The crab cyclin B cDNA was 3,794 bp containing an unusually long 3' untranslation region (UTR) of 2,403 bp and an open-reading frame encoding for a protein of 410 amino acids, with a calculated molecular mass of 45 kDa. The long 3'UTR harbors many cytoplasmic polyadenylation elements (CPE), and the GY-box, Brd-box, K-box that are perfectly complementary to the 5'-ends of various Drosophila microRNAs. The crab cyclin B transcript was predominantly expressed in ovary and testis. Semi-quantitative RT-PCR analysis revealed that the amount of cyclin B mRNA was high at previtellogenesis and late vitellogenesis stages, while low at early and middle vitellogenesis, suggesting that differential expression of cyclin B is closely related to oogonial proliferation (mitosis) and oocyte meiotic maturation.

• 出版日期2009-7
• 单位上海大学; 上海海洋大学