Higher order structure characterization of protein therapeutics by hydrogen/deuterium exchange mass spectrometry

作者：Huang Richard Y C; Chen Guodong^*

来源：Analytical and Bioanalytical Chemistry, 2014, 406(26): 6541-6558.

DOI：10.1007/s00216-014-7924-3

• Protein therapeutics
• Higher-order structure
• Mass spectrometry
• HDX-MS

Characterization of therapeutic drugs is a crucial step in drug development in the biopharmaceutical industry. Analysis of protein therapeutics is a challenging task because of the complexities associated with large molecular size and 3D structures. Recent advances in hydrogen/deuterium-exchange mass spectrometry (HDX-MS) have provided a means to assess higher-order structure of protein therapeutics in solution. In this review, the principles and procedures of HDX-MS for protein therapeutics characterization are presented, focusing on specific applications of epitope mapping for protein-protein interactions and higher-order structure comparison studies for conformational dynamics of protein therapeutics.

• 出版日期2014-10