Ultrasensitive Hydrogen Peroxide Biosensor Based on Enzyme Bound to Layered Nonoriented Multiwall Carbon Nanotubes/Polyelectrolyte Electrodes

作者：Munge Bernard S^*; Dowd Richard S; Krause Colleen E; Millord Lines N

来源：Electroanalysis, 2009, 21(20): 2241-2248.

DOI：10.1002/elan.200904676

• Nonoriented multiwall carbon nanotubes
• Horseradish peroxidase
• Biosensors
• Hydrogen peroxide
• Layer-by-layer
• Nanotubes

A sensitive hydrogen peroxide sensor based on horseradish peroxidase covalently attached to layered nonoriented MWNTs modified electrode is presented. Cyclic voltammetry results gave quasi-reversible Fe(III)/Fe(II) voltammetry. The electron transfer rate constant (k(s)) and Michaelis-Menten constant (K(M)) in pH 7 is 48.8 /- 0.9 s(-1) and 0.13 /- 0.05 mM respectively. A linear calibration curve for hydrogen peroxide was obtained up to 120 nM under the optimized conditions with a remarkable detection limit of (S/N=3) 1.5 nM. Results suggest that the nonoriented nanotubes act as electrical conductors and may also provide large surface area facilitating facile electron transfer and excellent electrochemical catalysis.

• 出版日期2009-10