An Immunosensor for alpha-Fotoprotein Antigen Based on Multi-wakked Nanotubes/L-Cysteine and Au@ Fe3O4 Nanocomposite

作者：Zhu Yu-Ping^*; Yuan Ruo; Chai Ya-Qing; Qin Song; Yuan Ya-Li

来源：Chinese Journal of Analytical Chemistry, 2012, 40(3): 359-364.

DOI：10.3724/SP.J.1096.2012.10735

• Ferriferrous oxide @ Au nanocomposites
• Carbon nanotubes
• L-Cysteine
• alpha-Fotoprotein
• Immunosensor

N, N-Dimethylfomamide(DMF) dispersed MWNTS was firstly modified on the electrode surface, which was in further eletrodeposited a nano-Au layer for immobilization of L-cysteine.Subsequently, Fe3O4 @ Au nanocomposites were absorbed on the modified electrode surface by the SH-Au bond for increasing the specific surface area and further immobilizing anti-AFP. Finally, bovine serum albumin (BSA) was used to block the non-specific adsorption sites of the immunosensor to obtain a highly sensitive and stable immunosensor. Experiments by transmission electron micros-copy (TEM) were carried out to characterize the prepared MWCNTs and Fe3O4 @ Au composite nanoparticles. Under optimal conditions,the sensor has a good response to AFP, in the detection range form 0.1 to 150 mu g/L, and detection limit of 0.03 ng/mL.

• 出版日期2012-3
• 单位内江师范学院