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摘要
In this study, development of icaritin-loaded liposomes stabilized by four kinds of surfactants (i.e. Span40, Span20, Sucrose ester and Tween80, with the hydrophile lipophile balance (HLB) numbers of 6, 8, 9 and 16, respectively) was presented, which tackled the poor solubility and low physicochemical stability of icaritin in the aqueous phase. The addition of icaritin and cholesterols into the bilayers exhibited an increase in the average diameter and high encapsulation efficiency, regardless of the variety of surfactants. Meanwhile, when cholesterols were absent, surfactants with low HLB numbers (<= 9) produced liposomes with higher average sizes (nearby 200 nm) and zeta-potentials (-20 to -35 mV) than Tween80. DPPF1* and ABTS"+ free radical-scavenging experiment exhibited different activities due to the diverse profiles of the free radicals, where their polarity and solubility must be taken into account. Physicochemical stability results revealed that Sucrose ester and Tween80 liposomal systems had the best physical and thermal stability, respectively. In addition, UV-vis absorption spectra and FTIR analysis confirmed that the formation of hydrogen bonds promoted the embedding effects of icaritin. Last but not the least, in vitro digestion experiment demonstrated that those liposomes containing Sucrose ester and Tween80 had the highest release levels, up to 75.68 +/- 0.25% and 77.23 +/- 0.58%, respectively. Therefore, these results suggested that the surfactants selection could be promising strategy for manufacturing different functional liposomal foods with different bioactive substance under various storage conditions.
- 出版日期2017-4-5
- 单位中国农业大学
