科研之友
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摘要
Heparin cofactor II (HCII) is a plasma serine protease inhibitor whose ability to inhibit alpha-thrombin is accelerated by a variety of sulfated polysaccharides in addition to heparin and dermatan sulfate. Previous investigations have indicated that calcium spirulan (Ca-SP), a novel sulfated polysaccharide, enhanced the rate of inhibition of alpha-thrombin by HCII. In this study, we investigated the mechanism of the activation of HCII by Ca-SP, Interestingly, in the presence of Ca-SP, an N-terminal deletion mutant of HCII (rHCII-Delta 74) inhibited alpha-thrombin, as native recombinant HCII (native rH-CII) did. The second-order rate constant for the inhibition of alpha-thrombin by rHCII-Delta 74 was 2.0 x 10(8) M-1 min(-1) in the presence of 50 mu g/ml Ca-SP and 10,000-fold higher than in the absence of Ca-SP, The rates of native rHCII and rHCII-Delta 74 for the inhibition of gamma-thrombin were increased only 80- and 120-fold, respectively, Our results suggested that the anion-binding exosite I of cu-thrombin was essential for the rapid inhibition reaction by HCII in the presence of Ca-SP and that the N-terminal acidic domain of HCII was not required. Therefore, we proposed a mechanism by which HCII was activated allosterically by Ca-SP and could interact with the anion-binding exosite I of thrombin not through the N-terminal acidic domain of HCII. The Arg(103) --> Leu mutant bound to Ca-SP-Toyopearl with normal affinity and inhibited alpha-thrombin in a manner similar to native rHCII, These results indicate that Arg(103) in HCII molecule is not critical for the interaction with Ca-SP.
- 出版日期2000-4-14
