A counting method for complex overlapping erythrocytes-based microscopic imaging

作者：Wei Xudong; Cao Yiping^*; Fu Guangkai; Wang Yapin

来源：Journal of Innovative Optical Health Sciences, 2015, 8(6): 1550033.

DOI：10.1142/S1793545815500339

• Cell counting
• image processing
• image segmentation
• overlap erythrocyte
• cell classification
• K-means

Red blood cell (RBC) counting is a standard medical test that can help diagnose various conditions and diseases. Manual counting of blood cells is highly tedious and time consuming. However, new methods for counting blood cells are customary employing both electronic and computer-assisted techniques. Image segmentation is a classical task in most image processing applications which can be used to count blood cells in a microscopic image. In this research work, an approach for erythrocytes counting is proposed. We employed a classification before counting and a new segmentation idea was implemented on the complex overlapping clusters in a microscopic smear image. Experimental results show that the proposed method is of higher counting accuracy and it performs much better than most counting algorithms existed in the situation of three or more RBCs overlapping complexly into a group. The average total erythrocytes counting accuracy of the proposed method reaches 92.9%.

• 出版日期2015-11
• 单位四川大学