Detection of Actinobacillus pleuropneumoniae through Duplex PCR Based on ApxIA and ApxIVA Genes

作者：Lin, Huayuan; Mao, Huirong; Cheng, Sufang; Yi, Mengying; Ruan, Yezhao; Wu, Cong; Xu, Zheng; Hu, Guoliang; Guo, Xiaoquan; Cao, Huabin; Li, Lin; Han, Yinhua; Liu, Ping^*

来源：Pakistan Veterinary Journal, 2018, 38(3): 276-280.

DOI：10.29261/pakvetj/2018.058

• Actinobacillus pleuropneumoniae
• ApxI
• ApxIV
• Duplex PCR

Actinobacillus pleuropneumoniae (APP) is the bacterial pathogen of pleuropneumoniae, a severe contagious and fatal respiratory disease of swine. For the purpose of setting up an easy way to detect this bacteria in swine, a duplex polymerase chain reaction (PCR) assay was built up for the detection of APP. Two pairs of primers were designed according to the APP ApxI and ApxIV gene sequences. Using these primers, a duplex PCR method was developed to fast identify APP in different bacteria samples, such as Enterococcus faecalis, Escherichia coli from cattle and swine, Staphylococcus aureus and Streptococcus. Our results have shown that duplex PCR could be a potential molecular method for the diagnosis of APP infection in swine.

• 出版日期2018
• 单位华南热带农业大学; 广西壮族自治区动物疫病预防控制中心; 江西农业大学; 华南农业大学