An update on using protein microarrays in drug discovery

作者：Kumble Krishnanand D^*

来源：Expert Opinion on Drug Discovery, 2007, 2(11): 1467-1476.

DOI：10.1517/17460441.2.11.1467

• activity-based probes
• antibody arrays
• antigen arrays
• protein microarrays
• protein-protein interactions
• surface plasmon resonance

Protein microarrays are evolving as useful tools for biopharmaceutical research. The differences in characteristics of individual proteins has made development challenging compared with DNA arrays. Nonetheless, significant advances have nontheless been made in developing protein microarray technology. Retention of function has been demonstrated for proteins belonging to various structural and functional classes after arraying. Focused arrays with small groups of proteins have been developed for a variety of applications, from biomarker validation to small molecule screening. Issues of protein stability as well as assay specificity and sensitivity, are being worked out for panels of arrayed proteins. The development of robust manufacturing methods has resulted in an increase in the number of commercially available protein array products. Quality control guidelines, which will also aid in accelerating development of the technology, are being established.

• 出版日期2007-11