A modified gelatin zymography technique incorporating total protein normalization

作者：Raykin Julia; Snider Eric; Bheri Sruti; Mulvihill John; Ethier C Ross^*

来源：Analytical Biochemistry, 2017, 521: 8-10.

DOI：10.1016/j.ab.2017.01.003

• Zymography
• Matrix metalloproteinases
• Loading controls
• Trihalocompounds
• Gelatinase

Gelatinase zymography is a commonly used laboratory procedure; however, variability in sample loading and concentration reduce the accuracy of quantitative results obtained from this technique. To facilitate normalization of gelatinase activity by loaded protein amount, we developed a protocol using the trihalocompound 2,2,2-trichloroethanol to allow for gelatin zymography and total protein labeling within the same gel. We showed that detected protein levels increased linearly with loading, and describe a loading concentration range over which normalized gelatinase activity was constant. We conclude that in -gel total protein detection is feasible in gelatin zymography and greatly improves comparison of gelatinase activity between samples. Published by Elsevier Inc.

• 出版日期2017-3-15