Bone-like nanocomposites based on self-assembled protein-based matrices with Ca2+ capturing capability

作者：Sang Lin; Huang Jie; Luo Dongmei; Chen Zhenhua; Li Xudong^*

来源：Journal of Materials Science: Materials in Medicine , 2010, 21(9): 2561-2568.

DOI：10.1007/s10856-010-4117-2

In the present work, bone-like nanocomposites have been successfully synthesized based on the mineralization of self-assembled protein-based microgels. Such mircogels were achieved by the in vitro reconstitution of collagen monomeric solutions in the presence of alginate in a microemulsion system. Microstructural observations revealed that the collagen-alginate composite beads possessed a nanofibrous three dimensional (3D) interconnected porous microstructure. The obtained microgels were pre-incubated in calcium-containing solution to capture Ca2+ ions, and subsequently immersed in phosphate-containing solution to initiate the formation of hydroxyapatite (HA) by an alternative incubating procedure. It was observed that a substantial amount of bone-like apatite nanocrystals were orderly and homogeneously deposited throughout the porous fibrillar networks. Herein, the collagen-alginate composite microgels served as a mineralization template for the synthesis of HA-polymer nanocomposites, which could be ideal vehicles potentially for cell carriers, bone repair and proteins and drugs delivery in tissue regeneration.

• 出版日期2010-9
• 单位四川大学