Development of Biological Nanopore Technique in Non-gene Sequencing Application

作者：Yang Jie; Li Shuang; Wu Xue-Yuan; Long Yi-Tao^*

来源：Chinese Journal of Analytical Chemistry, 2017, 45(12): 1766-1773.

DOI：10.1016/S1872-2040(17)61053-3

• Nanochannel
• Single-molecule detection
• Electrochemical analysis
• Ultralow current amplifier
• Review

Nanopore technique is a low-cost, ultrafast method for single-molecule level analysis without labels. Nanopore technique was first proposed more than 20 years ago and exhibited an excellent potential in DNA sequencing. So far, the commercial development of nanopore strand-sequencing as a portable device has been realized. Meanwhile, a remarkable number of studies have demonstrated that nanopores represent versatile single-molecule sensors for a wide range of molecules. Here, we mainly review the development of nanopore technique based on the interface interactions between biological pore and analytes such as proteins/peptides to obtain kinetic and thermodynamic structural information at single-molecule level. As examples, a large number of biological molecules and metal ions could be quantitatively detected by nanopore analysis, allowing its development for the future biotechnologies and medicine applications. Besides, the electrochemical measurement system is crucial for the nanopore technique. Therefore, we focus on advancements in relative software and ultralow current instrumentations with a high-bandwidth.

• 出版日期2017-12
• 单位华东理工大学