In vivo subcellular localization of Mal de Rio Cuarto virus (MRCV) non-structural proteins in insect cells reveals their putative functions

作者：Maroniche Guillermo A; Mongelli Vanesa C; Llauger Gabriela; Alfonso Victoria; Taboga Oscar; del Vas Mariana^*

来源：Virology, 2012, 430(2): 81-89.

DOI：10.1016/j.virol.2012.04.016

• MRCV
• Fijivirus
• Subcellular localization
• Non-structural proteins
• GFP
• Confocal microscopy

The in vivo subcellular localization of Mal de Rio Cuarto virus (MRCV, Fijivirus, Reoviridae) non-structural proteins fused to GFP was analyzed by confocal microscopy. P5-1 showed a cytoplasmic vesicular-like distribution that was lost upon deleting its PDZ binding TKF motif, suggesting that P5-1 interacts with cellular PDZ proteins. P5-2 located at the nucleus and its nuclear import was affected by the deletion of its basic C-termini. P7-1 and P7-2 also entered the nucleus and therefore, along with P5-2, could function as regulators of host gene expression. P6 located in the cytoplasm and in perinuclear cloudlike inclusions, was driven to P9-1 viroplasm-like structures and co-localized with P7-2, P10 and alpha-tubulin, suggesting its involvement in viroplasm formation and viral intracellular movement. Finally, P9-2 was N-glycosylated and located at the plasma membrane in association with filopodia-like protrusions containing actin, suggesting a possible role in virus cell-to-cell movement and spread.

• 出版日期2012-9-1