Lymphoid organ cell culture system from Penaeus monodon (Fabricius) as a platform for white spot syndrome virus and shrimp immune-related gene expression

作者：Jose S; Jayesh P; Sudheer N S; Poulose G; Mohandas A; Philip R; Singh I S Bright^*

来源：Journal of Fish Diseases, 2012, 35(5): 321-334.

DOI：10.1111/j.1365-2761.2012.01348.x

• BrdU assay
• immune-related genes
• immunofluorescence
• lymphoid cell culture
• Penaeus monodon
• white spot syndrome virus genes

Shrimp cell lines are yet to be reported and this restricts the prospects of investigating the associated viral pathogens, especially white spot syndrome virus (WSSV). In this context, development of primary cell cultures from lymphoid organs was standardized. Poly-l-lysine-coated culture vessels enhanced growth of lymphoid cells, while the application of vertebrate growth factors did not, except insulin-like growth factor-1 (IGF-1). Susceptibility of the lymphoid cells to WSSV was confirmed by immunofluoresence assay using monoclonal antibody against the 28 kDa envelope protein of WSSV. Expression of viral and immune-related genes in WSSV-infected lymphoid cultures could be demonstrated by RT-PCR. This emphasizes the utility of lymphoid primary cell culture as a platform for research in viruscell interaction, virus morphogenesis, up and downregulation of shrimp immune-related genes, and also for the discovery of novel drugs to combat WSSV in shrimp culture.

• 出版日期2012-5