Down-Regulation of microRNA-132 is Associated with Poor Prognosis of Colorectal Cancer

作者：Mokutani Yukako; Uemura Mamoru; Munakata Koji; Okuzaki Daisuke; Haraguchi Naotsugu; Takahashi Hidekazu; Nishimura Junichi; Hata Taishi; Murata Kohei; Takemasa Ichiro; Mizushima Tsunekazu; Doki Yuichiro; Mori Masaki; Yamamoto Hirofumi^*

来源：Annals of Surgical Oncology, 2016, 23(S5): S599-S608.

DOI：10.1245/s10434-016-5133-3

Background. Given the role of microRNA in colorectal cancer (CRC) progression, we explored the association between microRNA (miRNA) expression and CRC-related prognosis.
Methods. Three types of tissue samples (primary CRC lesions without liver metastasis, primary lesions with liver metastasis, and liver metastatic tissues) were used for miRNA profiling to identify differentially expressed miRNA. Quantitative real-time PCR was used to examine miRNA expression in CRC cells and in tumor tissues.
Results. MiR-132 was significantly down-regulated in primary CRC tissues with liver metastasis and liver metastatic lesions compared to primary lesions without liver metastasis. Multivariate analysis for overall survival indicated that low miR-132 expression was an independent prognostic factor for CRC patients (overall survival P = 0.040, disease-free survival P = 0.015). Ectopic expression of miR-132 significantly inhibited cell proliferation and cell invasion. The luciferase reporter assay revealed that anoctamin 1 (ANO1) was a direct target of miR-132. Kaplan-Meier survival curves showed that high ANO1 expression was a significant prognostic factor for overall survival of patients with CRC (P = 0.0344).
Conclusions. Down-regulation of miR-132 is associated with poor prognosis in CRC. ANO1 could be one of the crucial targets of miR-132 in CRC.

• 出版日期2016-12