Autologous Valve Replacement-CD133(+) Stem Cell-Plus-Fibrin Composite-Based Sprayed Cell Seeding for Intraoperative Heart Valve Tissue Engineering

作者：Kaminski Alexander^*; Klopsch Christian; Mark Peter; Yerebakan Can; Do***orf Peter; Gaebel Ralf; Eisert Friederike; Hasken Stefan; Kreitz Sebastian; Glass Aenne; Jockenhoevel Stefan; Ma Nan; Kundt Guenther; Liebold Andreas; Steinhoff Gustav

来源：Tissue Engineering - Part C: Methods , 2011, 17(3): 299-309.

DOI：10.1089/ten.tec.2010.0051

Objective: The development of biological valve prostheses with lifetime native-like performance and optimal host engraftment is an ultimate goal of heart valve tissue engineering. We describe a new concept for autologous graft coating based on a CD133(+)-stem-cells-plus-fibrin (SC+F) complex processed from bone marrow and peripheral blood of a single patient.
Methods: CD133(+)-SC (1 x 10(6) cells/mL) from human bone marrow and autologous fibrin (20mg/mL) were administered simultaneously via spray administration using the novel Vivostat Co-Delivery System. During static cultivation, SC+F performance was monitored for 20 days after delivery and compared with controls. For dynamic testing SC+F-composite was sprayed on a decellularized porcine pulmonary valve and transferred to a bioreactor under pulsatile flow conditions for 7 days.
Results: Static cultivation of SC+F-composite induced significant improvements in stem cell proliferation as compared with controls. For dynamic testing, microscopic analyses on a smooth engineered heart valve surface detected homogenous distribution of stem cells. Ultrasonic analysis revealed native-like valve performance. Applied CD133(+) stem cells differentiated into endothelial-like cells positive for CD31 and vascular endothelial growth factor receptor 2 and engrafted the valve. However, occasional delamination was observed.
Conclusion: SC+F serves as an excellent autologous matrix for intraoperative tissue engineering of valve prostheses promising optimal in vivo integration. However, stability remains an issue.

• 出版日期2011-3