An efficient transient mesophyll protoplast system for investigation of the innate immunity responses in the rubber tree (Hevea brasiliensis)

作者：Zhang, Xiaodong; Wang, Liangjie; He, Chaozu^*; Luo, Hongli^*

来源：Plant Cell, Tissue and Organ Culture, 2016, 126(2): 281-290.

DOI：10.1007/s11240-016-0997-2

• Rubber tree
• Mesophyll protoplast
• Transient expression
• Innate immunity responses

The transient mesophyll protoplast expression system has become a powerful tool for rapid gene functional analysis, and it has been used successfully in several plant species, including model plants Arabidopsis and rice, but not in rubber tree. In this study, we describe an improved method to isolate high quality rubber tree mesophyll protoplasts. The transient expression of exogenous DNA constructs in this mesophyll protoplast system was observed by using green fluorescent protein, and was detected by immunoblot. Furthermore, we used two typical pathogen-associated molecular patterns flg22 and chitin from bacteria and fungi, respectively, as inducers of plant innate immunity responses to detect the immunity response of rubber tree. Our data showed that flg22 and chitin induced activation of mitogen-activated protein kinases, generation and accumulation of reactive oxygen species, and transcription of defense genes. Our study demonstrated that the rubber tree mesophyll protoplast system can be used for functional analysis of immune responses of rubber tree and the dissection of cell signaling pathways. This transient mesophyll protoplast system may be applied to analyse gene function of rubber tree, a perennial plant for which the applicable transformation system was limited.

• 出版日期2016-8
• 单位海南大学