Distribution of alleles of grain quality genes in Indian bread wheat varieties

作者：Singh Anju M^*; Singh Santosh K; Ahlawat Arvind K; Jain Neelu; Singh G P; Ravi Indu; Yadav Mamata; Misra P C

来源：Indian Journal of Genetics and Plant Breeding, 2012, 72(2): 208-216.

• PINA
• PINB
• kernel texture
• PinaD1
• PinbD1
• serpins
• Srp5B
• flour yield

The prevalence of puroindoline hardness mutations (alleles) among Indian wheat varieties, germplasm lines, Triticum spelta and synthetic derivative accessions was surveyed in the present study. A total of 356 wheat genotypes were examined. While most T. spelta accessions had soft grains, very few among the rest of the lines were soft and possessed wild- type alleles of the two puroindoline genes. The majority were, however, uniformly hard. The null mutation of Pina and the wild type Pinb allele (Pina-D1b/Pinb-D1a) was the most frequent combination in the Indian wheat germplasm. Out of the 356 lines examined, only 42 possessed the Pinb-D1b allele indicating gly46serine mutation in Pinb. One landrace MPG33 possessed the Pinb-D1e allele as found by sequencing the 447 bp product obtained with Pinb-D1 primer in this line. Distribution of the alleles of the serpin (Srp5B) gene was also studied in 90 Indian wheat cultivars released during 1985-2010. Only 11 of the 90 genotypes possessed the b-type allele indicating the prevalence of the desirable a-type allele in the Indian wheat varieties. The role of the alleles of puroindoline and serpins genes in end-use quality of wheat is discussed.

• 出版日期2012-5