A Specific Process to Purify 2-Methyl-D-Erythritol-4-Phosphate Enzymatically Converted from D-Glyceraldehyde-3-Phosphate and Pyruvate

作者：Yang, Shao-Qing; Deng, Jian; Wu, Qian-Qian; Li, Heng; Gao, Wen-Yun^*

来源：Natural Product Communications, 2015, 10(2): 339-340.

DOI：10.1177/1934578x1501000233

• Methylerythritol phosphate pathway
• 2-Methyl-D-erythritol-4-phosphate
• Enzymatic cascade
• Purification

A one-pot enzymatic cascade was established to synthesize MEP, one of the key intermediates in the MEP terpenoid biosynthetic pathway. D-GAP and sodium pyruvate were converted to MEP in a reaction catalyzed by DXP synthase and DXP reductoisomerase (DXR) in the presence of the coenzymes ThPP, NADPH, and Mg2+. The product was then isolated by using a specific two-step purification process and MEP was obtained in a yield of nearly 60% and high purity. Importantly, MEP prepared by this way was totally free from contamination by minor amounts of DXP that was not completely convertible by DXR.

• 出版日期2015-2
• 单位西北大学