Accuracy and precision of a laser-spectroscopy approach to the analysis of delta H-2 and delta O-18 in human urine

作者：O'Grady Shannon P^*; Enright Lindsey E; Barnette Janet E; Cerling Thure E; Ehleringer James R

来源：Isotopes in Environmental and Health Studies, 2010, 46(4): 476-483.

DOI：10.1080/10256016.2010.536229

• cavity ring-down spectroscopy
• body water
• urine
• hydrogen-2
• isotope measurements
• methods and equipment
• oxygen-18
• infrared spectroscopy
• isotope application in medicine

The isotope ratio analysis of body water often involves large sample numbers and lengthy sample processing. Here we demonstrate the ability of isotope ratio infrared spectroscopy (IRIS) to rapidly and accurately analyse the isotope ratios of water in urine. We analysed water extracted from human urine using traditional isotope ratio mass spectrometry (IRMS) and compared those values with IRIS-analysed extracted water and un-extracted urine. Regression analyses for 2H and 18O values between (1) extracted water analysed via IRMS and IRIS and (2) urine and extracted water analysed via IRIS were significant (R2=0.99). These results indicate that cryogenic distillation of urine was not required for an accurate estimate of the isotopic composition of urine when using IRIS.

• 出版日期2010