A Spectroscopic Study of 2-[4'-(Dimethylamino)phenyl]-benzothiazole Binding to Insulin Amyloid Fibrils

作者：Kitts Catherine C; Bout David Anton Vanden^*

来源：Journal of Fluorescence, 2010, 20(4): 881-889.

DOI：10.1007/s10895-010-0634-0

• Amyloid fibrils
• 2-[4 '-(Dimethylamino)phenyl]-benzothiazole
• BTA-2
• Fluorescence
• Thioflavin T

The spectroscopic properties of 2-[4'-(dimethylamino)phenyl]-benzothiazole (BTA-2) in solution and in the presence of amyloid fibrils were investigated using absorption and fluorescence spectroscopy. Solution studies show that BTA-2 forms micelles in aqueous solutions, but that the dye can be solvated upon the addition of acetonitrile (CH(3)CN). BTA-2 binds to amyloid fibrils in solution leading to a characteristic blue-shift in the emission spectrum and an increase in fluorescence intensity. However, in solutions with increasing CH(3)CN concentration, there was a marked decrease in binding of the BTA-2 to fibrils. Studies demonstrating the effect of BTA-2 concentration on binding were performed. A comparison with the standard amyloid fluorescent marker, thioflavin T (ThT), showed that BTA-2 is more fluorescent, making it an excellent dye to label amyloid samples.

• 出版日期2010-7