科研之友
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摘要
Until now, many researchers have explained the degradation rate of biodegradable scaffold with respect to several parameters such as porosity, pore size, and strand diameter. In this study, to analyze the degradation rate of Polycaprolactone (PCL) scaffold, accelerated degradation experiment using sodium hydroxide (NaOH) was used. For the experiment, PCL scaffolds were fabricated by bioplotter with respect to porosity, pore size, and strand diameter, respectively. Each fabricated scaffold was put into a vial filled with 5 mol-NaOH 5 mL and trapped-air was removed using vacuum desiccator. After that, all vials were placed in the waterbath which was maintained with 37A degrees C For 24 days, seven vials were taken out from the waterbath for every 2 days and each scaffold was dried after rinsing with D.I. water. Afterwards, the degradation rate was analyzed for each type of PCL scaffolds using the measured mass. Among them, one type of scaffolds, which has the strand diameter of 300 mu m and the pitch between strands of 800 mu m, was used for the measurement of molecular weight change via gel permission chromatography (GPC). To show the each conventional parameter could not explain alone the degradation rate, the calculated degradation rates were analyzed with respect to porosity, pore size, and strand diameter, respectively. Afterwards, every degradation rate of all types of scaffolds was recorded with respect to the dimensionless surface area which is surface area/S-0. S-0 is the surface area of sphere which has same volume of respective scaffold. Consequently, the dimensionless surface area was found to be a single parameter irrelevant to the type of PCL scaffold to explain the in-vitro degradation rate of accelerated NaOH experiment.
- 出版日期2014-12
