Nanotopography promoted neuronal differentiation of human induced pluripotent stern cells

作者：Song Liqing; Wang Kai; Li Yan; Yang Yong

来源：Colloids and Surfaces B: Biointerfaces , 2016, 148: 49-58.

DOI：10.1016/j.colsurfb.2016.08.041

• Nanotopography
• Human induced pluripotent stem cells
• Neuronal differentiation
• YAP
• Extracellular matrix

Inefficient neural differentiation of human induced pluripotent stem cells (hiPSCs) motivates recent investigation of the influence of biophysical characteristics of cellular microenvironment, in particular nanotopography, on hiPSC fate decision. However, the roles of geometry and dimensions of nanotopography in neural lineage commitment of hiPSCs have not been well understood. The objective of this study is to delineate the effects of geometry, feature size and height of nanotopography on neuronal differentiation of hiPSCs. HiPSCs were seeded on equally spaced nanogratings (500 and 1000 nm in linewidth) and hexagonally arranged nanopillars (500 nm in diameter), each having a height of 150 or 560 nm, and induced for neuronal differentiation in concert with dual Smad inhibitors. The gratings of 560 nm height reduced cell proliferation, enhanced cytoplasmic localization of Yes-associated protein, and promoted neuronal differentiation (up to 60% (3III-tubulin+ cells) compared with the flat control. Nanogratinginduced cell polarity and cytoplasmic YAP localization were shown to be critical to the induced neural differentiation of hiPSCs. The derived neuronal cells express MAP2, Tau, glutamate, GABA and Islet-1, indicating the existence of multiple neuronal subtypes. This study contributes to the delineation of cellnanotopography interactions and provides the insights into the design of nanotopography configuration for pluripotent stem cell neural lineage commitment.

• 出版日期2016-12-1