Abundant LacZ activity in the absence of Cre expression in the normal and inflamed synovium of adult Col2al-Cre; ROSA26R(Lacz) reporter mice

作者：Fosang A J^*; Golub S B; East C J; Rogerson F M

来源：Osteoarthritis and Cartilage, 2013, 21(2): 401-404.

DOI：10.1016/j.joca.2012.11.013

• Col2a1
• Co12a1-Cre
• Cre-recombinase
• Synovium
• Inflammatory arthritis

Recent analyses of Col2al-Cre; ROSA26R reporter mice showed that synovial fibroblasts in 7-day mice were LacZ positive, due to a history of Col2al-Cre expression conferred by their origin in the interzone of the developing joint. We have examined LacZ staining in adult Col2a1-Cre(+10); ROSA26R(LacZ) mice, with and without inflammatory arthritis, and found that synovial fibroblasts in normal and inflamed synovium are LacZ positive, but Cre negative. Our results suggest that Cre-mediated recombination in joint interzone cells during development endure in adult synovial cells despite the absence of ongoing Cre expression. These findings have important implications and applications for the study of synovial inflammation in models of experimental arthritis.

• 出版日期2013-2