Determination of Angiotensin-(1-7) with HPLC/Fluorescence-Detection

作者：Russ Miriam; Hauser Susanne; Wintersteiger Reinhold; Greilberger Joachim; Andrae Michaela; Ortner Astrid^*

来源：Journal of Fluorescence, 2016, 26(1): 81-86.

DOI：10.1007/s10895-015-1686-y

• Angiotensin-(1-7)
• Fluorescamine
• HPLC
• Fluorescence detection
• Stability

Angiotensin-(1-7) is an important active component in the renin-angiotensin-system. Due to its cardio protective effects it is now under investigation in combination with antioxidants as a reperfusion solution. The combination showed impressive effects on isolated hearts of male Wistar rats after induced ischemia. In this work a high performance liquid chromatography method with fluorescence detection was developed for the first time for in-process measurements as well as for stability tests of the peptide in the novel antioxidant-containing KaralA (R) solution. For fluorescence detection of angiotensin-(1-7) fluorescamine as derivatization dye was applied. Under optimized conditions the method showed linearity over the range of 50 to 5000 ng/mL with R-2 of 0.9988 and an overall precision better than 5.0 %. LOD and LOQ were determined to be in the femtomol range on column. It was found that stability of angiotensin-(1-7) could be significantly improved in the antioxidant containing preparation compared to aqueous solutions.

• 出版日期2016-1