A FRET-based probe for fluorescence sensing of sulfide/sulfite analytes, using a novel long-wavelength water-soluble 7-hydroxycoumarin as reporter fluorophore

作者：Roubinet Benoit; Bailly Laetitia; Petit Emilie; Renard Pierre Yves; Romieu Anthony^*

来源：Tetrahedron Letters, 2015, 56(8): 1015-1019.

DOI：10.1016/j.tetlet.2015.01.042

• Coumarin dyes
• FRET probes
• SNAr
• Sulfhydryl analytes
• Water solubility

A FRET-based fluorescent probe for the detection of sulfide and/or sulfite in aqueous buffer, was constructed by connecting the quencher moiety nitrobenzofurazan (7-nitro-1,2,3-benzoxadiazole, NBD) to a water-soluble 3-(2-benzimidazoly1)-7-hydroxycoumarin carboxylic acid through a piperazine linker. This probe exhibits good selectivity and high sensitivity for sulfite and sulfide over cysteine and other potential analytes. Furthermore, this is one of the few examples of fluorogenic coumarins whose solubility in water is maintained upon its conversion into reaction-based fluorescent probes.

• 出版日期2015-2-18