Development and qualification of a size exclusion chromatography coupled with multiangle light scattering method for molecular weight determination of unfractionated heparin

作者：Beirne John; Truchan Hilary; Rao Lin^*

来源：Analytical and Bioanalytical Chemistry, 2011, 399(2): 717-725.

DOI：10.1007/s00216-010-4187-5

• Heparin
• Molecular weight and molecular weight distribution
• SEC/MALS
• dn/dc
• Second virial coefficient A(2)

The molecular weight of unfractionated heparin was determined by size exclusion chromatography (SEC) coupled with multiangle light scattering (MALS) detection. The SEC/MALS method determines absolute molecular weight directly from the angular dependence of scattered light intensity as a function of concentration and does not rely on molecular weight standards for column calibration. The SEC/MALS method developed at Scientific Protein Laboratories was qualified in terms of specificity, precision, robustness, and accuracy. By eliminating the requirement of well-characterized molecular weight standards derived from heparin, the present procedure represents a clear improvement over the column calibration methods used in molecular weight determination. The SEC/MALS method is suitable for routine quality control of unfractionated heparin.

• 出版日期2011-1