Astragalin attenuates lipopolysaccharide-induced inflammatory responses by down-regulating NF-kappa B signaling pathway

作者：Soromou Lanan Wassy; Chen Na; Jiang Lanxiang; Huo Meixia; Wei Miaomiao; Chu Xiao; Millimouno Faya Martin; Feng Haihua; Sidime Youssouf^*; Deng Xuming

来源：Biochemical and Biophysical Research Communications, 2012, 419(2): 256-261.

DOI：10.1016/j.bbrc.2012.02.005

• Astragalin
• Lipopolysaccharide
• Inflammatory responses
• Cytokine
• Endotoxemia
• Acute lung injury

Astragalin (AG), a flavonoid from many traditional herbs and medicinal plants, has been described to exhibit in vitro anti-inflammatory activity. The present study aimed to determine the protective effects and the underlying mechanisms of astragalin on lipopolysaccharide-induced endotoxemia and lung injury in mice. Mice were injected intraperitoneally (i.p.) with lipopolysaccharide (LPS) (dose range: 540 mg/kg). We observed mice on mortality for 7 days twice a day and recorded survival rates. In drug testing, we examined the therapeutic effects of astragalin (25, 50 or 75 mg/kg) on LPS-induced endotoxemia by dosing orally astragalin 1 hour before LPS challenge. Using an experimental model of LPS-induced acute lung injury (ALI), we examined the effect of astragalin in resolving lung injury. The investigations revealed that pretreatment with astragalin can improve survival during lethal endotoxemia and attenuate inflammatory responses in a murine model of lipopolysaccharide-induced acute lung injury. The mechanisms by which Astragalin exerts its anti-inflammatory effect are correlated with inhibition of tumor necrosis factor-a (TNF-alpha), interleukin-1 (IL-1), and interleukin-6 (IL-6) production via inactivation of NF-kappa B.

• 出版日期2012-3-9
• 单位吉林大学