Non-enzymatic electrochemical detection of glucose with a disposable paper-based sensor using a cobalt phthalocyanine ionic liquid graphene composite

作者：Chaiyo Sudkate; Mehmeti Eda; Siangproh Weena; Thai Long Hoang; Hai Phong Nguyen; Chailapakul Orawon^*; Kalcher Kurt^*

来源：Biosensors and Bioelectronics, 2018, 102: 113-120.

DOI：10.1016/j.bios.2017.11.015

• Paper-based analytical devices
• Non-enzymatic glucose sensor
• Cobalt phthalocyanine
• Graphene
• Ionic liquids

We introduce for the first time a paper-based analytical device (PAD) for the non-enzymatic detection of glucose by modifying a screen-printed carbon electrode with cobalt phthalocyanine, graphene and an ionic liquid (CoPc/G/IL/SPCE). The modifying composite was characterized by UV visible spectroscopy, energy dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The disposable devices show excellent conductivity and fast electron transfer kinetics. The results demonstrated that the modified electrode on PADs had excellent electrocatalytic activity towards the oxidation of glucose with NaOH as supporting electrolyte (0.1 M). The oxidation potential of glucose was negatively shifted to 0.64 V vs. the screen-printed carbon pseudo-reference electrode. The paper-based sensor comprised a wide linear concentration range for glucose, from 0.01 to 1.3 mM and 1.3-5.0 mM for low and high concentration of glucose assay, respectively, with a detection limit of 0.67 mu M (S/N = 3). Additionally, the PADs were applied to quantify glucose in honey, white wine and human serum. The disposable, efficient, sensitive and low-cost non-enzymatic PAD has great potential for the development of point-of-care testing (POCT) devices that can be applied in healthcare monitoring.

• 出版日期2018-4-15