Determination of aflatoxin B1 in rice, barley, and feed by non-instrumental immunochromatographic strip-test and high sensitive ELISA

作者：Shim Won Bo; Kim Jung Sook; Kim Ji Young; Choi Jin Gil; Je Jung Hyun; Kuzmina Nina Sergeevna; Eremin Sergei Alexandrovich; Chung Duck Hwa^*

来源：Food Science and Biotechnology, 2008, 17(3): 623-630.

• aflatoxin B1
• mycotoxin
• non-instrumental assay
• immunochrornatographic strip-test (ICG)
• enzyme-linked
• immunosorbent assay (ELISA)

A non-instrumental immunochromatographic (ICG) strip-test and direct competitive enzyme-linked immunosorbent assay (DC-ELISA) for aflatoxin B1 (AFB1) determination were developed and optimized. The detection limits of ICG strip-test and DGELISA were 0.5 and 0.004 ng/mL, respectively, and these methods possessed a cross-reaction to aflatoxins. The results of spiked samples by both inethods were coincided with the amount spiked AFB 1 and the comparative analyses of 172 real samples by 2 immunoassays and high performance liquid chromatography (HPLC) showed a good agreement. Especially, the lCG strip-test is easier to perform and quicker, but less sensitivity than DGELISA. Both inethods could analyze a high sample throughput with short time, but the sample throughput of ICG strip-test was better. Therefore, the lCG strip-test call be used as a simple, easy, non-instrumental, and fast screening technique for AFB1 determination.

• 出版日期2008-6