A novel microRNA miR-1165-3p as a potential diagnostic biomarker for allergic asthma

作者：Wu, Chaojie; Xu, Kun; Wang, Zhengxia; Chen, Zhongqi; Sun, Zhixiao; Yu, Wenqing; Ji, Ningfei^*; Huang, Mao^*; Zhang, Mingshun^*

来源：Biomarkers, 2019, 24(1): 56-63.

DOI：10.1080/1354750X.2018.1501762

• Asthma
• miR-1165-3p
• allergic rhinitis (AR)
• allergic bronchopulmonary aspergillums (ABPA)
• biomarker
• Th2

Context: A further examination of a novel miRNA,miR-1165-3p as a biomarker for asthma, which was previously implicated in helper T cells (Th2) in a murine asthma model.Objective: To determine whether serum miR-1165-3p can serve as a potential diagnostic biomarker for allergic asthma.Methods: Serum miR-1165-3p was quantified via quantitative real-time PCR (qRT-PCR) in asthmatic and control samples. Serum miR-1165-3p levels were compared between groups and the clinical diagnostic abilities of miR-1165-3p were evaluated. The analyses utilized included a student's t test, one-way ANOVA, and the generation of receiver operating characteristic (ROC) curves.Results: Serum miRNA-1165-3p levels were significantly elevated in asthmatics when compared to the healthy controls. Furthermore, the sensitivity and specificity of serum miR-1165-3p were found to be 83% and 68.2%. Additionally, serum miR-1165-3p levels were also found to be significantly elevated in patients with allergic rhinitis (AR) or allergic bronchopulmonary aspergillosis (ABPA).Conclusions: This study showed that serum miR-1165-3p can potentially be utilized as a noninvasive biomarker that is able to aid in the diagnosis and characterization of allergic asthma.

• 出版日期2019-1-2
• 单位南京医科大学