Alternative splicing of a viral mirtron differentially affects the expression of other microRNAs from its cluster and of the host transcript

作者：Rasschaert Perrine; Figueroa Thomas; Dambrine Ginette; Rasschaert Denis; Laurent Sylvie

来源：RNA Biology, 2016, 13(12): 1310-1322.

DOI：10.1080/15476286.2016.1244600

• Cluster of microRNAs
• long non-coding RNA
• Marek's disease virus
• mirtron
• splicing

Interplay between alternative splicing and the Microprocessor may have differential effects on the expression of intronic miRNAs organized into clusters. We used a viral model the LAT long non-coding RNA (LAT lncRNA) of Marek's disease oncogenic herpesvirus (MDV-1), which has the mdv1-miR-M8-M6-M7-M10 cluster embedded in its first intron to assess the impact of splicing modifications on the biogenesis of each of the miRNAs from the cluster. Drosha silencing and alternative splicing of an extended exon 2 of the LAT lncRNA from a newly identified 3 splice site (SS) at the end of the second miRNA of the cluster showed that mdv1-miR-M6 was a 5-tailed mirtron. We have thus identified the first 5-tailed mirtron within a cluster of miRNAs for which alternative splicing is directly associated with differential expression of the other miRNAs of the cluster, with an increase in intronic mdv1-miR-M8 expression and a decrease in expression of the exonic mdv1-miR-M7, and indirectly associated with regulation of the host transcript. According to the alternative 3SS used for the host intron splicing, the mdv1-miR-M6 is processed as a mirtron by the spliceosome, dispatching the other miRNAs of the cluster into intron and exon, or as a canonical miRNA by the Microprocessor complex. The viral mdv1-miR-M6 mirtron is the first mirtron described that can also follow the canonical pathway.

• 出版日期2016