A novel and efficient immobilised tannase coated by the layer-by-layer technique in the hydrolysis of gallotannins and ellagitannins

作者：Crestini Claudia^*; Lange Heiko

来源：Microchemical Journal, 2015, 123: 139-147.

DOI：10.1016/j.microc.2015.05.025

• Tannase
• Enzyme-immobilisation
• Layer-by-layer coating
• Gallotannins
• Ellagitannins
• P-31 NMR

Tannase (EC 3.1.1.20) was quantitatively immobilised onto Eupergit (R) C 250 Land coated by layers of alternatively charged polystyrene sulfonate and polyallylamine hydrochloride, respectively. The layer-by-layer (LbL)-coated immobilised tannase, Le., LbL-tannase retained its original activity and showed significant resistance to deactivation and maintained 50% of its activity after seven consecutive cycles of hydrolysis reactions, each run for 24 h. The LbL coating did not hinder the substrate access to the active site of the enzyme. Both gallo- and ellagitannins were efficiently hydrolysed upon treatment with LbL-tannase. The formation of gallic acid and the reaction patterns were followed by HPLC and the recently developed P-31 NMR analytical method for the characterisation of tannins.

• 出版日期2015-11