Novel phenol biosensor based on laccase immobilized on reduced graphene oxide supported palladium-copper alloyed nanocages

作者：Mel Li Ping; Feng Jiu Ju; Wu Liang; Zhou Jia Ying; Chen Jian Rong; Wang Ai Jun^*

来源：Biosensors and Bioelectronics, 2015, 74: 347-352.

DOI：10.1016/j.bios.2015.06.060

• Alloyed nanocages
• Reduced graphene oxide
• Laccase
• Biosensor
• Catechol

Developing new nanomaterials is of key importance to improve the analytical performances of electrochemical biosensors. In this work, palladium-copper alloyed nanocages supported on reduced graphene oxide (RGO-PdCu NCs) were facilely prepared by a simple one-pot solvothermal method. A novel phenol biosensor based on laccase has been constructed for rapid detection of catachol, using RGO-PdCu NCs as electrode material. The as-developed phenol biosensor greatly enhanced the electrochemical signals for catechol. Under the optimal conditions, the biosensor has two linear ranges from 0.005 to 1.155 mM and 1.655 to 5.155 mM for catachol detection at 0.6 V, the sensitivity of 12.65 mu A mM(-1) and 5.51 mu A mM(-1), respectively. This biosensor showed high selectivity, low detection limit, good reproducibility, and high anti-interference ability.

• 出版日期2015-12-15
• 单位浙江师范大学