Development of Clostridium difficile R20291 Delta PaLoc model strains and in vitro methodologies reveals CdtR is required for the production of CDT to cytotoxic levels

作者：Bilverstone T W; Kinsmore N L; Minton N P^*; Kuehne S A

来源：Anaerobe, 2017, 44: 51-54.

DOI：10.1016/j.anaerobe.2017.01.009

• C. difficile
• PaLoc
• Binary toxin
• CdtR
• Virulence

Assessing the regulation of Clostridium difficile transferase (CDT), is complicated by the presence of a Pathogenicity locus (PaLoc) which encodes Toxins A and B. Here we developed R20291 Delta PaLoc model strains and cell-based assays to quantify CDT-mediated virulence. Their application demonstrated that the transcriptional regulator, CdtR, was required for CDT-mediated cytotoxicity.

• 出版日期2017-4