Acetobacter musti sp nov., isolated from Bobal grape must

作者：Ferrer Sergi^*; Manes Lazaro Rosario; Benavent Gil Yaiza; Yepez Alba; Pardo Isabel

来源：International Journal of Systematic and Evolutionary Microbiology, 2016, 66(2): 957-961.

DOI：10.1099/ijsem.0.000818

An acetic acid bacterium (strain Bo7(T)), obtained during a study of the microbial diversity of spontaneous fermentations of Bobal grape must, was subjected to a taxonomic study using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences allocated strain Bo7(T) to the genus Acetobacter, and revealed Acetobacter aceti and Acetobacter oeni to be nearest neighbours (99.57 % 16S rRNA gene sequence similarity between strain Bo7(T) and A. oeni CECT 5830(T), and 98.76 % between strain Bo7(T) and A. aceti CECT 298(T)). Cells of strain Bo7(T) are Gram-negative, motile rods, catalase-positive and oxidase-negative. The DNA G+C content of strain Bo7(T) was 58.0 mol%. DNA-DNA hybridizations demonstrated that strain Bo7(T) belongs to a single novel genospecies that can be differentiated from its nearest phylogenetic neighbours by the following phenotypic characteristics: no production of 5-keto-D-gluconic acid from D-glucose, growth with glycerol but not with methanol or maltose as sole carbon sources, and growth on yeast extract with 30 % D-glucose. The major fatty acid was C-18 : 1 omega 7c/C-18 : 1 omega 6c (summed feature 8; approx. 56 %); other fatty acids in significant amounts (>5 %) were C-16 : 0 2-OH (11 %), C-16 : 0 (7 %), C-14 : 0 2-OH (7 %) and C-14 : 0 3-OH/iso-C-16 : 1 I (summed feature 2; 6 %). The results obtained indicate that strain Bo7(T) represents a novel species of the genus Acetobacter, for which the name Acetobacter musti sp. nov. is proposed. The type strain is Bo7(T) (=DSM 23824(T)=CECT 7722(T)).

• 出版日期2016-2