A conditional mouse line for lineage tracing of Sox9 loss-of-function cells using enhanced green fluorescent protein

作者：Chatterjee Sumantra; Kraus Petra; Sivakamasundari V; Xing Xing; Yap Sook Peng; Jie Song; Lufkin Thomas^*

来源：Biotechnology Letters, 2013, 35(12): 1991-1996.

DOI：10.1007/s10529-013-1303-6

• Col2a1
• Conditional knockout
• Embryogenesis
• Enhanced green fluorescent protein (EGFP)
• Mouse mutant
• Sox9
• Stem cells

Traditionally, conditional knockout studies in mouse have utilized the Cre or Flpe technology to activate the expression of reporter genes such as lacZ or PLAP. Employing these reporter genes, however, requires tissue fixation. To make way for downstream in vivo or in vitro applications, we have inserted enhanced green fluorescent protein (EGFP) into the endogenous Sox9 locus and generated a novel conditional Sox9 null allele, by flanking the entire Sox9 coding region with loxP sites and inserting an EGFP reporter gene into the 3%26apos;-UTR allowing for EGFP to be expressed upon Sox9 loss of function yet under the control of the endogenous Sox9 promoter. Mating this new allele to any Cre-expressing line, the fate of Sox9 null cells can be traced in the cell type of interest in vivo or in vitro after fluorescence-activated cell sorting.

• 出版日期2013-12