Development of a real-time RT-PCR method for rapid detection of H9 avian influenza virus in the air

作者：Lv, Jing; Wei, Baozhi; Chai, Tongjie^*; Xia, Xianzhu; Miao, Zengmin; Yao, Meiling; Gao, Yuwei; Huang, Rong; Yang, Huili; Roesler, Uwe

来源：Archives of Virology, 2011, 156(10): 1795-1801.

DOI：10.1007/s00705-011-1054-4

• A VIRUS
• TRANSMISSION
• SUBTYPE
• CHINA
• EVOLUTION
• CHICKENS
• POULTRY
• EPIDEMIOLOGY
• SYSTEM
• ASSAY

Avian influenza virus (AIV) has caused serious epidemics all over the world. Notably, the low-pathogenic AIV H9N2 has been spreading widely, leading to enormous economic losses to the poultry industry. To rapidly monitor airborne H9 AIVs in chicken houses, a real-time RT-PCR method was established and used to detect virus in air samples, and it was also compared with the traditional RT-PCR. The results showed that the real-time RTPCR possessed high specificity and sensitivity for H9 AIVs, and the sensitivity reached 100 copies/reaction, much higher than the traditional RT-PCR; airborne H9 AIVs were found in the six chicken houses by real-time RT-PCR, and their mean concentrations ranged from 1.25x10(4) to 6.92x10(4) copies/m(3) air. Overall, the real-time PCR is a valuable tool for detecting airborne H9 AIVs.

• 出版日期2011-10
• 单位山东农业大学; 中国疾病预防控制中心