Near-infrared optical-resolution photoacoustic microscopy

作者：Hai Pengfei; Yao Junjie; Maslov Konstantin I; Zhou Yong; Wang Lihong V^*

来源：Optics Letters, 2014, 39(17): 5192-5195.

DOI：10.1364/OL.39.005192

Compared with visible light (700 nm), near-infrared light (1400 nm) undergoes weaker optical attenuation in biological tissue; thus, it can penetrate deeper. Herein, we demonstrate near-infrared optical-resolution photoacoustic microscopy (NIR-OR-PAM) with 1046 nm illumination. A penetration depth of 3.2 mm was achieved in chicken breast tissue ex vivo using optical fluence within the American National Standards Institute (ANSI) limit (100 mJ/cm(2)). Beyond similar to 0.6 mm deep in chicken breast tissue, NIR-OR-PAM has shown finer resolution than the visible counterpart with 570 nm illumination. The deep imaging capability of NIR-OR-PAM was validated in both a mouse ear and a mouse brain. NIR-OR-PAM of possible lipid contrast was explored as well.

• 出版日期2014-9-1