Studies on purification and bioactivity of 17400 shark cartilage angiogenesis inhibitor

作者：Zeng F^*; Yang WX; Cui KY; Liu W; Zhang ZX; Yu JX; Pang Y

来源：Chemical Journal of Chinese Universities, 2003, 24(9): 1588-1591.

• shark cartilage
• angiogenesis inhibitor
• separarion and purification
• bioactivity
• chorioallantoic membrane of chick embryos

SCAI-c, another angiogenesis inhibitor from shark cartilage of Guangdong Yangjiang, was purified and the bioactivity of SCAI-c were investigated. The 1.0 mol/L guanidine and 0.02 mol/L MES(at 28 degreesC) extracts of shark cartilage was centrifuged at 6 000 r/min for 30 min and the pellet was discarded, and the supernatant was recentrifuged at 12 000 r/min for 30 min, then ultrafiltrated and fractionally precipitated with 25%-90% acetone. The precipitate was applied to a Sephadex G-75 gel column and eluted after the predominand peak of A(280 nm) absorbing material with 0.05 mol/L Tris-HCl buffer (containing 0.02 mol/L NaCl and 0.02% NaN3). The material B were composed of three single peptides with molecular mass of 17 600, 15 000, 12 600, then were purified by using reverse-phase HPLC, the eluate of C was collected, dried and observed by chicken chorioallantoic membrane. The pure inhibitor was homogeneous as a single band on a silver-stained 12.5 % sodium dodecyl sulfate-polyacrylamide gel. SCAI-c has a molecular weight of 17 600, and could specifically inhibit angiogenesis of the chorioallantoic membrane of chick embryos, and the inhibitory effect of angiogenesis was SCAI-c concentration dependent, but the concentration of SCAI-c increased to 0. 5 mug/muL, chick embryos died about to 20%-30%. Inhibitory characteristic of SCAI-c was similar with that of SCAI-a. They are specific angiogenesis inhibitor, and may be a useful drug on tumor therapy in some day.

• 出版日期2003-9-10
• 单位中山大学