Mitochondrial Efficiency-Dependent Viability of Saccharomyces cerevisiae Mutants Carrying Individual Electron Transport Chain Component Deletions

作者：Kwon Young Yon; Choi Kyung Mi; Cho ChangYeon; Lee Cheol Koo

来源：Molecules and Cells, 2015, 38(12): 1054-1063.

DOI：10.14348/molcells.2015.0153

• cellular ATP
• electron transport chain
• ETC-component single gene deletion
• mitochondria
• mitochondrial ROS

Mitochondria play a crucial role in eukaryotic cells; the mitochondrial electron transport chain (ETC) generates adenosine triphosphate (ATP), which serves as an energy source for numerous critical cellular activities. However, the ETC also generates deleterious reactive oxygen species (ROS) as a natural byproduct of oxidative phosphorylation. ROS are considered the major cause of aging because they damage proteins, lipids, and DNA by oxidation. We analyzed the chronological life span, growth phenotype, mitochondrial membrane potential (MMP), and intracellular ATP and mitochondrial superoxide levels of 33 single ETC component-deleted strains during the chronological aging process. Among the ETC mutant strains, 14 (sdh1 Delta, sdh2 Delta, sdh4 Delta, cor1 Delta, cyt1 Delta, qcr7 Delta, qcr8 Delta, rip1 Delta, cox6 Delta, cox7 Delta, cox9 Delta, atp4 Delta, atp7 Delta, and atp17 Delta) showed a significantly shorter life span. The deleted genes encode important elements of the ETC components succinate dehydrogenase (complex II) and cytochrome c oxidase (complex IV), and some of the deletions lead to structural instability of the membrane-F1F0-ATP synthase due to mutations in the stator stalk (complex V). These short-lived strains generated higher superoxide levels and produced lower ATP levels without alteration of MMP. In summary, ETC mutations decreased the life span of yeast due to impaired mitochondrial efficiency.

• 出版日期2015-12