Antimicrobial and Cytotoxic Isohexenylnaphthazarins from Arnebia euchroma (Royle) Jonst. (Boraginaceae) Callus and Cell Suspension Culture

作者：Damianakos Harilaos; Kretschmer Nadine; Syklowska Baranek Katarzyna; Pietrosiuk Agnieszka; Bauer Rudolf; Chinou Ioanna^*

来源：Molecules, 2012, 17(12): 14310-14322.

DOI：10.3390/molecules171214310

• Arnebia euchroma
• Boraginaceae
• callus culture
• cell suspension culture
• isohexenylnaphthazarins
• cytotoxic
• antimicrobial

The phytochemical investigation of the n-hexane extract from callus and cell suspension culture of Arnebia euchroma (Royle) Jonst. resulted in the isolation of nine isohexenylnaphthazarins: deoxyalkannin (1), alkannin (2), acetylalkannin (3), isobutyrylalkannin (4), beta-hydroxyisovalerylalkannin (5), 2 %26apos;%26apos;-(S)-alpha-methylbutyrylalkannin (6), propionylalkannin (7), teracrylalkannin (8) and acetylshikonin (9). Their structures were determined by MS and NMR spectroscopy. Pigments 2-8 are isolated for the first time from Arnebia in vitro cultures, 4 and 7 are reported in the present work as novel metabolites within the Arnebia genus, while 9 is a known constituent of both natural roots and in vitro cultures of A. euchroma. Moreover, methyl jasmonate and 1-monoglyceryl olate, palmitate and stearate are reported for the first time within the Boraginaceae family. The antimicrobial and cytotoxic activities of all isolated pigment compounds were tested, revealing a very interesting profile.

• 出版日期2012-12