Association of high HLA-E expression during acute cellular rejection and numbers of HLA class I leader peptide mismatches with reduced renal allograft survival

作者：Guberina Hana^*; Rebmann Vera; Wagner Bettina; Nardi Fabiola da Silva; Dziallas Phillip; Dolff Sebastian; Bienholz Anja; Wohlschlaeger Jeremias; Bankfalvi Agnes; Heinemann Falko M; Witzke Oliver; Zoet Yvonne M; Claas Frans H J; Horn Peter A; Kribben Andreas; Doxiadis Ilias I N

来源：Immunobiology, 2017, 222(3): 536-543.

DOI：10.1016/j.imbio.2016.10.021

• Renal transplantation
• Acute cellular rejection
• HLA-E
• NKG2C
• HLA class I leader peptides
• Adaptive immunity

Non-classical Human Leukocyte Antigen (HLA)-E preferentially presents leader peptides derived from classical HLA-class I molecules. HLA-E can trigger opposed immune responses by interacting with inhibitory NKG2A or by activating NKG2C receptors on NK and T-cells. We studied the impact of HLA-E on renal allograft survival during acute cellular rejection. HLA-E expression was up-regulated in acute cellular rejection (ACR) biopsies (n = 12) compared to biopsies from 13 renal allografts with no rejection-signs. HLA-E up-regulation was correlated with numbers of HLA-class I leader peptide mismatches (p = 0.04). CD8+ and CD56+ infiltrating cells correlated with HLA-E expression (p <0.0001 and p = 0.0009, respectively). Activating NKG2C receptor dominated on effector cells in biopsies and peripheral blood during ACR potentially allowing HLA-E-mediated immune activation. Moreover, HLA-E expression correlated with deterioration in renal allograft p <0.008) and reduced allograft survival (p = 0.002). Our findings provide evidence that during renal allograft rejection HLA-E along with high numbers of mismatched HLA-class I leader peptides might represent additional targets for immune-activating responses.

• 出版日期2017-3