Autophagy and oxidative stress associated with gold nanoparticles

作者：Li Jasmine J; Hartono Deny; Ong Choon Nam; Bay Boon Huat^*; Yung Lin Yue L

来源：Biomaterials, 2010, 31(23): 5996-6003.

DOI：10.1016/j.biomaterials.2010.04.014

• Gold nanoparticles
• Oxidative stress
• Autophagy
• Autophagosomes
• Lung fibroblasts

Elemental metal nanoparticles like cadmium and silver are known to cause oxidative stress and are also highly toxic. Yet for gold nanoparticles (AuNPs), it is not well established whether these particles are biologically toxic. Here we show that AuNPs, which were taken up by MRC-5 human lung fibroblasts in vitro, induce autophagy concomitant with oxidative stress. We also observed formation of autophagosomes together with the uptake of AuNPs in the lung fibroblasts as well as upregulation of autophagy proteins, microtubule-associated protein 1 light chain 3 (MAP-LC3) and autophagy gene 7 (ATG 7) in treated samples. AuNP treated cells also generated significantly more lipid hydroperoxides (p-value <0.05), a positive indication of lipid peroxidation. Verification with western blot analysis for malondialdehyde (MDA) protein adducts confirmed the presence of oxidative damage. In addition, AuNP treatment also induced upregulation of antioxidants, stress response genes and protein expression. Exposure to AuNPs is a potential source of oxidative stress in human lung fibroblasts and autophagy may be a cellular defence mechanism against oxidative stress toxicity.

• 出版日期2010-8