Fast and sensitive determination of aluminium with RP-HPLC using an ultra-short monolithic column

作者：Remenyi Tomas A; Nesterenko Pavel N^*; Bowie Andrew R; Butler Edward C V; Haddad Paul R

来源：Analytical Methods, 2011, 3(11): 2488-2494.

DOI：10.1039/c1ay05192j

A fast and sensitive reversed-phase high-performance liquid-chromatographic method for determination of aluminium in aqueous samples has been developed. The fluorescent aluminium-lumogallion complex (lambda(ex) 505 nm, lambda(em) 574 nm), was formed with a pre-column reagent and then separated on a Chromolith (R) RP-18e Guard column using a two-tiered, stepped gradient program, with matrix elimination (5/95 (v/v) methanol/water), followed by elution (90/10 (v/v) methanol/water). This method achieved a run time of 2.5 min without compromising sensitivity (limit of detection = 5.6 x 10(-10) M, limit of quantification = 7.2 x 10(-7) M), precision (3.5% at 3.71 x 10(-6) M) or accuracy (recovery = 97.5% +/- 3.2%, n = 5, P = 0.95). This represents an improvement in run time by >50% compared to the fastest previously published method [Lee et al., Clin. Chem., 1996, 42, 1405-1411]. This method uses MES buffer (2-(N-morpholino) ethanesulfonic acid), which compared to other buffers is more easily purified, does not complex with aluminium, and can be used in lower concentrations. This method was applied to analysis of: deionised water for column comparison; seawater for matrix interference effects; and tea-infusion for calibration and recovery studies.

• 出版日期2011-11