科研之友
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摘要
Conclusion: Claudius%26apos; cells absorb Na+. through the amiloride-sensitive epithelial sodium channel (ENaC). Transepithelial ion transport through ENaC and possibly a Cl- secretory pathway is regulated by P2Y purinergic signaling. Objectives: The purpose of this study was to investigate ion transport in Claudius%26apos; cells and its purinergic regulation. Methods: Young adult Sprague-Dawley rats and gerbils were studied. The Claudius%26apos; cell layer on the basilar membrane was dissected from the basal turn of the cochlea. A voltage-sensitive vibrating probe was used to measure transepithelial short circuit current (I-sc). The baseline I-sc of Claudius%26apos; cells was measured in the perilymph-like control solution and the change of I-sc after application of amiloride (10 mu M) or uridine triphosphate (UTP, 100 mu M). Results: A negative baseline I-sc was observed in the control solution (-12.50 +/- 3.95 mu A/cm(2), n = 8) and the addition of amiloride resulted in a decrease of I-sc by 75.8%. The application of UTP, an agonist for P2Y purinergic receptors, led to a partial inhibition of I-sc (by 38.2 +/- 3.2%, n = 5), and subsequent addition of amiloride abolished the remaining I-sc.
- 出版日期2012-6
