An alkali-halotolerant cellulase from Bacillus flexus Isolated from green seaweed Ulva lactuca

作者：Trivedi Nitin; Gupta Vishal; Kumar Manoj; Kumari Puja; Reddy C R K^*; Jha B

来源：Carbohydrate Polymers, 2011, 83(2): 891-897.

DOI：10.1016/j.carbpol.2010.08.069

• Cellulose
• Cellulase
• Halo alkali tolerance
• Marine habitat

An extracellular alkali-halotolerant cellulase from the strain Bacillus flexus NT isolated from Ulva lactuca was purified to homogeneity with a recovery of 25 03% and purity fold of 22 31 The molecular weight of the enzyme was about 97 kDa and the V(max) and K(m) was 370 17 U/ml/min and 6 18 mg/ml respectively The optimum pH and temperature for enzyme activity was 10 and 45 C respectively The enzymatic hydrolysis of the CMC was confirmed with GPC and GC-MS analysis The stabilized activity of the enzyme even at high pH of 9 0-12 0 and residual activity of about 70% at salt concentration (NaCl 15%) revealed for its alkali-halotolerance nature The metal ions Cd(2+) and Li(1+) were found as inducers while Cr(2+) Co(2+) Zn(2+) and metal chelator EDTA have signi

• 出版日期2011-1-10
• 单位海洋化工研究院有限公司